WebClonExpress Ⅱ One Step Cloning Kit Catalog # C112 Simple, fast, efficient and suitable for any vector Does not depend on ligase, since even the phenomenon of carrier-free, positive rate of upto 95% Efficiently clone 50 bp-10 kbp DNA fragment Linearized cloning vector and PCR products were not purified may direct cloning Without regard to ... WebCatalog # C112 The ClonExpress cloning technology is simple, fast, and high efficient which enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly, the vector is linearized at the cloning site. A small sequence overlapped with each end of the cloning site is added onto the insert through PCR.

ClonExpress II One Step Cloning Kit – hybridea

WebProduct Description. This product is the second-generation TOPO cloning kit, which contains the second-generation Topoisomerase, a vector containing the suicide gene ccdB, and a blunt-end factor. Using the second-generation Topoisomerase, with the most suitable buffer, it has higher activity and further improves the cloning efficiency. WebThe ClonExpress II is a novel cloning Kit, independent of DNA ligase, significantly reducing the vself-ligated colonies and bringing a true positive rate > 95%. The enhanced Exnase II and highly optimized buffer significantly improve the recombination efficiency and the tolerance to impurities.Both the linearized vector and the PCR products of ... tretinoin and led light therapy

2 × Vazyme LAmp Master Mix (Dye Plus) P312

WebRapid PCR 2 × Rapid Taq Master Mix P222... WebFawn Creek KS Community Forum. TOPIX, Facebook Group, Craigslist, City-Data Replacement (Alternative). Discussion Forum Board of Fawn Creek Montgomery County … WebClonExpress ® technology is a simple, fast and efficient DNA seamless cloning technology, which can clone the insert into any position of any vector. Linearize the vector and place the insert 5'in the forward/reverse PCR primer Introduce the end sequence of the linearized vector into the end, so that the 5'and 3'ends of the PCR product have the same … ten commandments prince buster