WebMar 7, 2013 · This method leads to an efficient characterization of the binding free-energy surface and an accurate calculation of the absolute protein–ligand binding free energy. We illustrate our protocol in two systems, benzamidine/trypsin and SC-558/cyclooxygenase 2. In both cases, the X-ray conformation has been found as the lowest free-energy pose ... WebAug 6, 2024 · In addition, the limited dynamic range of visual readouts of gels that are often used to evaluate pull-down experiments increases the danger of misinterpretation or overinterpretation of these experiments. Overall, observing binding in pull-downs and related experiments is a complex function of the experimental components and conditions.
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