Collagen IV (ColIV or Col4) is a type of collagen found primarily in the basal lamina. The collagen IV C4 domain at the C-terminus is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, collagen IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause the collagen to form in a sheet, the form of the basal lamin… WebWe use pCas9_GFP (Addgene plasmid 44719) to induce DSBs and pCas9D10A_GFP to induce paired nicks (nCAS9) (Addgene plasmid 44720), together with sgRNA expression plasmids derived from MLM3636 (Addgene plasmid 43860). 2. Competent bacteria, e.g., DH5α. 3. LB agar plates with antibiotic (ampicillin for the plasmids above). 4. LB medium. 5.
Endothelial cell-specific collagen type IV-α3 expression does not ...
WebApr 15, 2024 · The glomerular basement membrane (GBM) is a critical component of the kidney’s blood filtration barrier. Alport syndrome, a hereditary disease leading to kidney … WebNov 11, 2024 · Collagen accumulates in the ER in SEC24-depleted cells. Sec24d KO MEFs were transfected with indicated siRNAs (50 nM) at day 0. The culture medium was replaced with fresh medium at day 1. ... (Addgene, Watertown, MA, USA) to generate a GFP-SEC24D fusion gene. GFP-SEC24D sequence was amplified with PCR using primers … immortals 2011 plot
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WebApr 15, 2024 · Collagen type IV-α 4 (COL4A4) was not detected in induced Col4a3 −/− transgenic (Tg) mice. Kidneys from Col4a3 −/− Tg mice and Col4a3 +/− control mice (78 … WebLearn about depositing plasmids with Addgene, and find answers to questions about Deposit Agreements and Rewards Points. Viral Preps and Antibodies. Learn about … WebUse BD BioCoat™ Collagen I 100 mm culture dishes. Day 0. Transfection. I. ... 4. Pipet gently to mix completely. 5. Add TransIT®-293 Reagent to the diluted pDNA mixture. 6. Pipet gently to mix completely. 7. Incubate at room temperature for 15-30 min. 8. Add 600 ul TransIT-293®-DNA complex dropwise to different areas of the plate. immortals 2015